User Training

All request are to be submitted via the PPMS booking system: Newcomers get ICP and PPMS accounts during their onboarding. For external visitors and interns we need to know the following before we can organize a training for you:

  1. You will need to provide a cost account number, including an approval from your PI (and grant office in case you want to speed up matters)
  2. Please check if you can log in to our booking system.  In case you can not yet log in, you can request an account by sending an email to ppms@ist.ac.at and include all info from point 1
  3.  Request a training via the booking system: see “Getting Started – section
  • Visiting scientists & interns: please have your visiting lab assist you with the points listed above. (see “visitors and guests”  section below *)
  • In case you need a different introduction than listed, please contact us by e-mail: bioimaging@ist.ac.at. and include information as listed at point 1 and 2.

GETTING STARTED:

1 – Introduction to new users: all new users of the Bioimaging Facility need to complete the “introduction to new users “, before they can make use of any facility equipment. We will provide explanations about our i) usage & booking guidelines, ii) system trainings, iii) booking system (PPMS/BIF),  and iv) safety guidelines (laser, mercury lamps).  Please sign up for the ‘New user introduction to Bioimaging Facility’ here.

2 – System trainings: Before any system can be used, one needs to complete a system training provided by facility staff. Each training, as a rule, consists of at least two parts (see training policy below). During these training sessions, Please submit a system training request form for the equipment you want to training for  via our  booking system.

3 – Application trainings: next to system trainings, we provide assistance for trail experiments and/or trainings for i) advanced imaging, ii) cell cytometry and iii) image analysis. Please submit a assistance request form for assistance via our  booking system.


USER TRAINING POLICY

Training, as a rule, consists of two parts:

The 1st introduction is a basic training where we allow up to 1 person at the same time. When you request it, you will need to prepare for your intro according to the technique you want to use. You can find links to the reading materials that will introduce you to the basics of the system that we offer below on this page. Please do not be surprised, if during the training we will ask you some questions based on these links.

The 2nd part of the training must happen within maximum period of two weeks after the first part, for only 1 person at a time. You will need to provide your own sample and you will be asked to operate the instrument in our presence. We will help you to set up your imaging experiment and can discuss your image post-processing workflow.

During the 2nd training, we will evaluate to what extend you are able to operate the instrument. We will give you a permission to book this instrument on your own in case we feel you can work with the microscope safely and independently. Please understand that if we have the feeling that you are not very secure with the instrument, we might ask you to repeat the training session. Only after both training sessions are successfully completed will we grant you permission to book instruments on your own.

Rights expire after prolonged ‘no use’ (9 months – 1 year): a refresher training will be required to regain user rights.


* VISITORS and GUESTS:

We can introduce external guests, visiting scientists & interns for 2 systems max (minimal required stay = 2 months). Please inform us at least 2 weeks in advance, so we can organize your training timely. You will need to provide a cost account with approval from your PI and the name of one group member of the visiting lab, who will be your internal contact person.


MICROSCOPY TRAINING MATERIALS

In order to improve impact of training that are given by BioImaging Facility team members, we kindly ask you to study materials listed below, before the scheduled introduction takes place. BioImaging Facility staff will ask you questions based on these materials and will only start training if these questions are answered adequately.


For all BioImaging Facility devices, please review following materials:

http://zeiss-campus.magnet.fsu.edu/articles/basics/introduction.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/imageformation.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/resolution.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/psf.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/opticaltrain.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/kohler.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/opticalsystems.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/fluorescence.html

Additionally, for the following groups of devices, materials listed below have to be reviewed and understood:


Confocal microscopes (Leica SP5, Leica SP8, LSM800, LSM880):

https://www.microscopyu.com/techniques/confocal/introductory-confocal-concepts

Multi-photon microscopes (LaVision upright, inverted):

https://www.microscopyu.com/techniques/multi-photon/multiphoton-microscopy

SD laser cutter, iMIC spinning disc:

http://zeiss-campus.fsu.edu/articles/spinningdisk/introduction.html

Widefield (Nikon Eclipse, Visitron TIRF, Olympus widefield)

http://zeiss-campus.magnet.fsu.edu/articles/basics/contrast.html

TIRF (Imic TIRF, Visitron TIRF, Olympus TIRF):

https://www.microscopyu.com/techniques/fluorescence/total-internal-reflection-fluorescence-tirf-microscopy

SPIM (OpenSPIM):

http://openspim.org/Welcome_to_the_OpenSPIM_Wiki

Atomic Force Microscopy:

https://www.bruker.com/products/surface-and-dimensional-analysis/atomic-force-microscopes


Flow cytometers (BD FACS Canto II and FACS Aria III)

https://www.bdbiosciences.com/eu/s/training/e-learning/e-learningcourses


ADDITIONAL RESOURCES

Please also consider watching some of the seminars provided by the iBiology platform:

https://www.ibiology.org/online-biology-courses/microscopy-series/


 

We are very grateful for the resources provided on these external websites, as listed above!

BIF team