User trainings

GETTING STARTED:

TRAININGS: Before anyone can make use of any system, one needs to complete a training from the Bio-Imaging Facility team. Each training, as a rule, consists of two parts (see training policy below). During these trainings, you will also be instructed with our usage & booking guidelines and the safety guidelines (laser, mercury lamps).

To get a training:  you need to submit a training request via our online booking system (PPMS).

To get a PPMS account: Newcomers get ICP and PPMS accounts during their onboarding. For external visitors and interns we need to know the following before we can organize a training for you:

  1. You will need to provide a cost account number, including an approval from your PI (and grant office in case you want to speed up matters)
  2. Please check if you can log in to our booking system.  In case you can not yet log in, you can request an account by sending an email to ppms@ist.ac.at and include all info from point 1
  3. Please request a training via the booking system: please book here
  • For visiting scientists and interns: please have your visiting lab assist you with the points listed above.
  • In case you need a different introduction than listed, please contact us by e-mail: bioimaging@ist.ac.at. and include information as listed at point 1 and 2.

USER TRAINING POLICY

Training, as a rule, consists of two parts:

The 1st introduction is a basic training where we allow up to 2 people at the same time. When you request it, you will need to prepare for your intro according to the technique you want to use. You can find links to the reading materials that will introduce you to the basics of the system that we offer below on this page. Please do not be surprised, if during the training we will ask you some questions based on these links.

The 2nd part of the training must happen within maximum period of two weeks after the first part, for only 1 person at a time. You will need to provide your own sample and you will be asked to operate the instrument in our presence. We will help you to set up your imaging experiment and can discuss your image post-processing workflow.

During the 2nd training, we will evaluate to what extend you are able to operate the instrument. We will give you a permission to book this instrument on your own in case we feel you can work with the microscope safely and independently. Please understand that if we have the feeling that you are not very secure with the instrument, we might ask you to repeat the training session. Only after both training sessions are successfully completed will we grant you permission to book instruments on your own.

VISITORS and GUESTS:

We can introduce external guests, visiting scientists & interns for 2 systems max (minimal required stay = 2 months). Please inform us at least 2 weeks in advance, so we can organize your training timely. You will need to provide a cost account with approval from your PI and the name of one group member of the visiting lab, who will be your internal contact person.


MICROSCOPY TRAINING MATERIALS

In order to improve impact of training that are given by BioImaging Facility team members, we kindly ask you to study materials listed below, before the scheduled introduction takes place. BioImaging Facility staff will ask you questions based on these materials and will only start training if these questions are answered adequately.


For all BioImaging Facility devices, please review following materials:

http://zeiss-campus.magnet.fsu.edu/articles/basics/introduction.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/imageformation.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/resolution.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/psf.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/opticaltrain.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/kohler.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/opticalsystems.html

http://zeiss-campus.magnet.fsu.edu/articles/basics/fluorescence.html

Additionally, for the following groups of devices, materials listed below have to be reviewed and understood:


Confocal microscopes (Leica SP5, Leica SP8, LSM800, LSM880):

https://www.thorlabs.com/tutorials.cfm?tabID=8ed9f76d-3bbd-4e6c-b2f5-f6a89a2c4fc9

Multi-photon microscopes (LaVision upright, inverted):

https://www.thorlabs.com/tutorials.cfm?tabID=8ed9f76d-3bbd-4e6c-b2f5-f6a89a2c4fc9

SD laser cutter, iMIC spinning disc:

http://zeiss-campus.fsu.edu/articles/spinningdisk/introduction.html

Widefield (Nikon Eclipse, Visitron TIRF, Olympus widefield)

http://zeiss-campus.magnet.fsu.edu/articles/basics/contrast.html

TIRF (Imic TIRF, Visitron TIRF, Olympus TIRF):

https://www.microscopyu.com/techniques/fluorescence/total-internal-reflection-fluorescence-tirf-microscopy

SPIM (OpenSPIM):

http://openspim.org/Welcome_to_the_OpenSPIM_Wiki

Atomic Force Microscopy:

https://www.bruker.com/products/surface-and-dimensional-analysis/atomic-force-microscopes


Flow cytometers (BD FACS Canto II and FACS Aria III)

https://www.bdbiosciences.com/eu/s/training/e-learning/e-learningcourses


ADDITIONAL RESOURCES

Please also consider watching some of the seminars provided by the iBiology platform:

https://www.ibiology.org/online-biology-courses/microscopy-series/


 

We are very grateful for the resources provided on these external websites, as listed above!

BIF team