Usage Policy: Cytometry

GENERAL:

All guidelines described  for ‘microscopy’ also apply for the use of our flow cytometry equipment:

  • All systems need to be booked before use: see booking system
  • All users need a training by the BioImaging Facility staff before they can book a specific system
  • It is strictly forbidden to use any of the setups without booking or training
  • It is strictly forbidden to use any of the setups on the name or booking of another user
  • Bookings can not be made longer than 14 days in advance

In addition:

  • FACS service can only be provided within the core facility time  of IST: 9:00 till 5:00 pm Monday – Thursday, and 9:00 – 15:30 on Friday.
  • To ensure availability of a FACS service (novice assistance), your reservation needs to be made minimal two working days in advance of your experiment (i.e., Monday for Wednesday etc.). Only when service is booked in timely in advance, the BioImaging Facility can guarantees that service will be provided. (Weekend days count as one day- i.e. , you have to book on Friday to ensure service on Monday.). Make sure to book assistance using the correct forms in ppms: Request assistance by BIF staff for Flow Cytometry

The following rules apply for the BD FACS Aria III:

  1. Startup and shutdown of the machines is performed by BioImaging Facility staff only: we cannot make exceptions, as they result in down-times and additional costs.
  2. FACS Aria III service can only be provided within the core facility time  of IST: 9 am till 5:30 pm Monday-Thursday, 9 am till 15:30 pm Friday.
  3. To ensure availability of a FACS service, your reservation needs to be made minimal two working days in advance of your experiment (i.e., Monday for Wednesday etc.). Only when service is booked in timely in advance, the BioImaging Facility can guarantees that service will be provided. (Weekend days count as one day- i.e. , you have to book on Friday to ensure service on Monday.)
  4. If FACS service is booked on shorter term, we TRY to match your needs, but cannot guarantee it. The reason is, that our FACS experts are also providing other services and have to plan their time in advance.
  5. Cancelling FACS bookings within 24 hours prior to slot start is only possible via email to bioimaging@ist.ac.at
  6. In case late sorts (past 6 p.m.) or weekend sorts are required: we cannot guarantee them. In case there is a SERIOUS experimental demand on this, we can discuss it on case-by-case basis and look for a solution
  7. If your configuration (nozzle size, temperature or sample collection rack) differs from that of the previous user or if you are the first user at the time of booking, please make sure you always select the ‘Set sorting configurations by BIF’ checkbox in your booking. This is needed to ensure enough time before your slot to allow us to reconfiguration the sorter for your experiment– otherwise we’ll have to do it in your session!

The following rules apply for the Sony SH800-SFP:

  1. Upon successful training for the device, users have to complete 5 sessions without the need of help/assistance from facility staff in order to get autonomous booking rights (out of hours and weekends) for the sorter.
  2. All users have to provide their own (or their own groups) sample line and sorting chips. We strongly advise you to always have a backup chip at hand.
  3. As with all other equipment: assistance can be provided by BIF facility staff during office hours. Please request it at least 48h prior to your slot by email to bioimaging@ist.ac.at