Location: I06.U1.403
Axio Imager.Z2 Upright LSM800 confocal microscope + Airyscan, suited for live imaging: equipped with heating chamber, CO2 + O2 mixer, ZEN 2.6 with experiment designer and macro-enviroment (OAD)
This confocal microscope is well suited for life imaging: book in PPMS– 4h limit in core hours
Overnight acquisitions: Please reboot system before and start system in DEBUG MODE (+ screenshot script): do not forget to SAVE the debug log-file in case of a crash!!
Sample conditioning:
- Heating chamber
- NO CO2 mixer
Light sources:
- Laser lines 405, 488, 561, 640 nm
- CoolLED p300 SB light
- ESID LED
Detectors: 3 channels simultaneously (more sequentially)
- 2x GaAsP PMTs: Gallium arsenide phosphide photomultiplier tube detectors with free choice of the spectral range. (Uses a tuneable short-pass and band-pass filter in front of the PMTs)
- 1 x Airyscan detector – optimized for 63x. Can also be used as 3rd GaAsP detector!
- 1x ESID+LED light source: Electronically switchable illumination and detection module
Motorized stage for multiple position imaging
- Motorized stage for multiple position imaging
- Multi-slide holder, dish holder, single slide holder
Objectives (M27 thread)
- Plan APOCHROMAT 20x/0.8 (420650-9901), WD=0.55 mm (D=0.17 mm)
- Plan-Apochromat 10x/0.45 (420640-9900), WD=2.1 mm
- Plan-Apochromat 40x/1.3 Oil DIC, UV-IR (420762-9800-799), WD=0.20 mm (Airyscan)
- Plan-Apochromat 20x / NA 1.0 W DIC (WD=1.8mm), (UV) VIS-IR
Not mounted objectives:
- Objective Plan-Apochromat 63x / NA 1.4 Oil
- Plan APOCHROMAT 40x/1.1 Water
- Plan APOCHROMAT 5x/0.16
Software: Zen 2.6/ HotFix8
- ZEN Module Experiment Designer Hardware
- ZEN Module Tiles & Positions
- ZEN Macro Editor
Computer: HP Z6
- Intel® Xeon™ E5-2623 v3 CPU @ 3.0GHz + 2GHz (2 processors)
- 64GB RAM
- 3.38TB data drive
Contact persons:
Manual:
See detailed description and manual: LSM800 upright-02 MANUAL_2019_08_06