Olympus IX83 TIRF

Location:

I04.02.031

Total Internal Reflection (TIRF) microscope from Olympus (Japan)

  • Olympus IX83 inverted microscope equipped with a Cell^TIRF-4-line module
  • motorized stage for multiple position imaging
  • Incubator and CO2 supply for live cell imaging

Multicolor TIRF with independent angle control

The Olympus cellTIRF-4Line system enables ultra-sensitive, simultaneous multicolor TIRF using four laser channels with independent beam paths. Individual motorized angle control for each laser ensures equal evanescent penetration (±1 nm), which enables the user to produce high-contrast images with minimal background noise for cell surface and single molecule studies.

Since the angle of each laser source is independently motorized, their penetration depths can be synchronized across each wavelength using cellSens software. This also provides key data about the penetration depths that are possible with each laser/objective pair.

Scheme of the Cell^TIRF-4-line system

light sources:

  • CW Diode Lasers, laser lines 405 nm, 488 nm, 561 nm, 640 nm
  • Transmitted light

filter sets:

  • Quadfiltercube: zt405-488-561-640rpc from Chroma (USA) and FF01-446/523/600/677-25 from Laser2000 (UK)
  • GFP filter cube: LP491; BP 525/45

camera:

  •  Hammamatsu ImagEM X2 EM-CCD: C9100-13, (512×512 pixels, pixel size = 16µm)

software:

cellSens Dimension 2.2

dual channel splitting optics:

W-VIEW GEMINI image splitting optics from Hammamatsu (Japan) provide one pair of dual-wavelength images separated by a dichroic mirror onto a single camera.

Filter options are: Green (524/27) /Red (617/73); Green (525/50) /FarRed (700/75); Red (579/34)/FarRed(700/75)

Dichroic mirrors: T580; T640



Objectives

  1. Olympus UAPON OTIRF 100x /1.40 OIL for TIRF imaging
  2. Olympus Uplan SApo 100x/1.40 OIL
  3. UAPON 0340 40xOIL available for brightfield and widefield fluorescence modes only !!!
  4. TIRF Tool 0.5x for alignment purposes, BIF Only !!!

contact persons:

 

See detailed description and manual