Nikon CSU-W1-01 SoRa+NIR

Location: room i04.U1.015 – book in PPMS


The Nikon CSU W1-01 SoRa+NIR spinning disk microscope is an advanced enhanced resolution imaging modality, and the future (!) platform of the 355 nm UV laser ablation system. It has a piezo drive for fast and precise imaging of 3D stacks, and is equipped with an incubation chamber and a CO2/O2 gas mixer.


Imaging modalities/features

  1. Confocal (spinning disk)
  2. Widefield (CoolLED)
  3. Super resolution via optical Re-assignment (SoRa)
  4. NIR imaging (not available in SoRa mode)
  5. Feedback microscopy

Specifications

  • Nikon Ti2E inverted microscope (double deck) with Perfect Focus System (PSF) / widefield
  • Yokogawa CSU-W1 dual-disk spinning disk unit with 50 μm pinholes & 50 μm pinholes with micro-lenses SoRa disk
  • Yokogawa SoRa with magnification changer for optimum resolution across different objective magnifications (for SoRa modality)
    • 4.0x; 60x objective; 55×55 μm FOV (40x objectives also possible with limited resolution gain)
    • 2.8x; 100x objective; 48×48 μm FOV
    • 1.0x; normal spinning disk imaging
    • Resolution
  SoRa mode (optical only) SoRa mode + deconvolution
XY resolution 150 nm 120 nm
Z resolution 320 nm 300 nm
  • 2 Teledyne Photometrics BSI cameras
  • Motorized stage:
    1. Nikon TI2-S-SE-E XY stage with linear encoders: 100 nm XY resolution, 500 nm XY accuracy
    2. Mad City Labs Z piezo & linear stage encoder: travel range: 200 μm, 0.4 nm min step size
  • Incubation chamber: IST custom-built dark chamber with Life Imaging Services heating unit (25-42 ºC)
  • Ibidi CO2/O2 gas mixer

Light sources

  • CoolLED – pE-300 White Light LED source
  • Visible lasers: Omicron LightHUB UltraN
    • 405 nm laser:  (120 mW) – Continuous wave
    • 488 nm laser:  (200 mW) – Continuous wave
    • 561 nm laser:  (150 mW) – Continuous wave
    • 638 nm laser:  (200 mW) – Continuous wave
  • NIR laser: Omicron LightHUB NIR
    • 785 nm laser:  (200 mW) – Continuous wave

Filters for visual/widefield examination (lower deck)

  • DAPI-5060C Filter Cube: Ex 377/50, DM 409, Emission BP 447/60
  • GFP-4050B Filter Cube: Ex 466/40, DM 495, Emission BP 525/50
  • mCherry-C Filter Cube: Ex 562/40, DM 593, Emission BP 640/75
  • Cy5-4040C Filter Cube: Ex 628/40, DM 660, Emission BP 692/40

Objectives / position

  1. CFI Plan Apo λ 10x air / NA 0.45 / WD 4 mm (MRD00105)
  2. CFI Plan Apo λ 40x air / 0.95/ 0.17-0.25 mm (MRD00405)
  3. CFI Plan Apo VC 60x WI/ NA 1.2 / WD 0.28-0.31 mm / Water – (MRD07602) – €€
  4. CFI Plan Apo λ 60x oil / NA 1.4/  WD 0.13 mm / oil (MRD01605) – €€
  5. CFI SR HP Plan Apo λ 100x Sil / NA 1.35/ WD 0.30 mm / Silicone oil (MRD73950) – €€€
  6. Free slot for bookable objectives
 
Additional high-end bookable objectives (book in PPMS)
  1. CFI Apo LWD λ S 20XC WI / NA 0.95 / WD 0.9-0.99 mm / Water (MRD77200) – €€€

Facility objective (UV test BIF)

CFI Plan-Apo λ 40x / NA 0.75 / WD 0.66 mm (MRH00401)


Light path and cameras

image source

Dichroic Mirror Block

  1.  Confocal (visible lasers) quad-band dichroic mirror: 405 nm / 488 nm / 561 nm / 640 nm
  2. Widefield (CoolLED) dichroic mirror: 350-700 nm
  3. Near InfraRed (NIR laser) dichroic mirror: 785 nm

Image Splitting Dichroic

  1. DM A561 nm Long Pass (FITC / TRITC-dual camera)
  2. Glass (transmits to camera 2)
  3. Mirror (reflects to camera 1)

Emission filter wheel camera 1 (Photometrics BSI)

  1. quad-band 440/521/607/700 nm → DAPI / FITC / TRITC / Cy5
  2. single bandpass: 447/60 nm  → DAPI / BFP
  3. single bandpass: 525/30 nm  → GFP, Venus, mNeonGreen, Alexa488, FITC
 Emission Filter wheel Camera 2 (Photometrics BSI)
  1. quad-band 440/521/607/700 nm → DAPI / FITC / TRITC / Cy5
  2. single bandpass: 600/52 nm → TRITC, mCherry
  3. single bandpass: 708/75 nm → Cy5
  4. single bandpass: 835/70 nm → NIR

Cameras: 2 x Teledyne Photometrics BSI (USB3) sCMOS camera, 95% quantum efficiency; 6.5 µm x 6.5 µm pixel area


Computer (HP Z8 Workstation): 

Dual Intel Xeon 4114 2.2 2400 MHz 10 CPU

RAM: 128 GB DDR4 2666 MHz

Nvidia Quadro RTX5000

2 TB SSD drive for fast imaging

6 TB HDD

10 GB Ethernet card

 

Manual


Contact:

Yann Cesbron