Location: room i04.U1.015 – book in PPMS
The Andor Dragonfly is an advanced imaging platform with multiple imaging modalities, Borealis Perfect Illumination, incubation chamber and CO2/O2 mixers.
Imaging modalities/features:
- Confocal (dual spinning disk with 25 & 40 um pinhole)
- Laser-based Widefield
- Total Internal Reflection (TIRF) Microscopy
- Super-resolution (SRRF)
- Astigmatic lens for 3D SMLM (single-molecule localization microscopy)
Specifications:
- Nikon Ti2 inverted microscope (single deck)
- 2 cameras: 1x Andor iXon EMCCD and 1x Andor Zyla sCMOS
- Spinning disk with two pinhole patterns: 25 µm and 40 µm in confocal mode
- Camera zoom optics of 1.0x, 1.5x and 2.0x for optimum resolution across different objective magnifications and camera pixel sizes
- Illumination zoom optics of 4x, 16x & 36x power density
- Motorized stage:
- ASI XY stage: the resolution of 100 nm for XY-axis
- ASI Z-piezo & linear stage encoder: Linear XY encoders are available to provide 50 nm resolution – Z positions with sub-nanometre accuracy (travel-range: 500 microns)
- Incubation chamber (25-42 ºC)
- Ibidi CO2/O2 mixer
Light sources:
- CoolLED – pE-300 White Light source
- 405 nm laser: (100 mW) – Continuous-wave
- 488 nm laser: (150 mW) – Continuous-wave
- 561 nm laser: (150 mW) – Continuous-wave
- 637 nm laser: (140 mW) – Continuous wave
Filter for visual examination:
- DAPI: Excitation: BP 377/50 Emission: BP 447/60
- GFP/FITC: Excitation: BP 482/18 Emission: BP 520/28
- DsRed/RHOD: Excitation: BP 531/40 Emission: BP 593/40
Objectives / position:
- CFI P-Apo 10x Lambda / NA 0.45 / WD 4.0 mm (MRD00105)
- CFI P-Apo 20X Lambda / NA 0.75 / WD 1.00 mm (MRD00205)
- CFI P-Apo 60x Lambda / NA 1.40 / WD 0.13 mm – oil (MRD01605)
- CFI Apochromat TIRF 60x / NA 1.49 / WD 0.13 mm – oil (MRD0169) – €€€
- Apochromat LWD λS 40x / 1.15 Water 0.60 mm (MRD77410) – €€€
- Open position (avilable for high-end rental objectives)
Extra high-end Objectives (Available via Automated rental System)
- CFI P-Apochromat 10x NA 0.50 / WD 5.50 mm – Glycerol (MRD71120) – €€€ → book seperately (rights required – info)
- CFI P-Apochromat 20x NA 0.95 / WD 0.95 mm – Water (MRD77200) – €€€ → booke seperately (rights required – info)
- Other available objectives
Lightpath and cameras
Cameras:
- Andor iXon Ultra 888 Ultra EMCCD Camera. Active Pixels 1024 x 1024; Pixel Size 13 x 13 μm; Image Area (mm) 13.3 x 13.3;
- Andor Zyla 4.2 Megapixel sCMOS
Camera Emission Splitter (for 2nd Port camera)
- Dual camera switching mirror: use to switch emission light path between primary and secondary camera ports
- Dual camera dichroic 565 nm longpass: for dual colour GFP/RFP simultaneous imaging (shorter wavelength is reflected to vertical camera port)
- Dual camera dichroic 640 nm longpass: for dual colour Cy3/Cy5 simultaneous imaging (shorter wavelength is reflected to vertical camera port)
Emission Filter wheel Camera 1 (Andor iXon)
- 445/46 bandpass filter: DAPI / BFP / Blueberry / Azurite (use with dichroics #1, 2 and 6 only)
- 521/38 bandpass: GFP, Venus, mNeonGreen, Alexa488, FITC, mWasabi. For use with dichroics # 1, 2 and 6 only.
- 594/43 bandpass: RFP/TRITC, mRuby, tDTomato. (use with dichroics #1, 5 and 6)
- 620/60 bandpass: mCherry, Hc-Red1, mRuby
- 685/47 bandpass: Cy5, Cy5.5, iRFP 670/682, mCardinal (use with dichroic #6 only)
- Dual EM filter 488-561 – GFP/RFP
- 478/37 band pass: CFP, mCerulean, mTurquoise, DAPI. For use with dichroics #3, 4 and 5 only.
- Penta EM filter 405-488-561- 640: (DAPI/GFP/RFP/Cy5)
Emission Filter wheel Camera 2 (Andor Zyla)
- 445/46 bandpass filter: DAPI / BFP / Blueberry / Azurite (use with dichroics #1, 2 and 6 only)
- 538/20 bandpass filter: YFP, Venus, mCitrine. (use with dichroic #5 only)
- 521/38 bandpass: GFP, Venus, mNeonGreen, Alexa488, FITC, mWasabi. For use with dichroics # 1, 2 and 6 only.
- 594/43 bandpass: RFP/TRITC, mRuby, tDTomato. (use with dichroics #1, 5 and 6)
- 620/60 bandpass: mCherry, Hc-Red1, mRuby
- 685/47 bandpass: Cy5, Cy 5.5, iRFP 670/682, mCardinal (use with dichroic #6 only)
- Dual EM filter 488-561 – GFP/RFP
- Penta EM filter 405-488-561- 640: (DAPI/GFP/RFP/Cy5)
Computer (Fusion-Imaris workstation PC):
- Idual quad-core Xeon CPUs
- RAM: 96GB ECC
- nVidia M4000 8GB
- AMD W7100 8GB
- 6x SSDs (4TB for images)
- 10 Terabyte disk drive upgrade.
Manual
A detailed description and manual: DRAGONFLY-01
More information: microscope feature description (ReasearchGate)
Contact persons: