Equipment & Resources

The Bioimaging Facility is facilitating the use and maintenance of an wide variety of advanced Microscopy, Imaging analysis and Cytometry devices, as well as software licenses and system accessories. Before anyone can make use of any system, users need to complete a training by the Bio-Imaging Facility team before bookings can be made.

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• usage policy
• booking policy
• safety guidelines
• data storage policy

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i. EQUIPMENT PER PRODUCT GROUP

CYTOMETRY

00. Cell analysis & cell sorting

1. BD CANTO II analyzer (room i04.EG.017): RETIRED
2. BC Cytoflex LX Cell Analyser (room i04.EG.017): Coming soon (2021)
3. BD Aria III Cell sorter (room i04.EG.017): book in PPMS
4. SONY SH800 Cell sorter (room i04.EG.017): book in PPMS
5. FlowJo (remote): book in PPMS

MICROSCOPY

01. Widefield Microscopes

1. Olympus VS120 slide scanner (room i06.U1.406): book in PPMS
2. Nikon Eclipse Ti inverted (room i04.U1.018): RETIRED
3. Nikon Eclipse Ti2E-01 inverted (room i06.U1.406): book in PPMS
4. Nikon Eclipse Ti2E-02 inverted (room i04.U1.015): book in PPMS
5. Zeiss Axioscope (room i06.U1.403): book in PPMS
6. Biolumenescence imaging (room i04.U1.018):
7. Neurolucida (room i04.U1.018): RETIRED

02. Confocal Microscopes

1. Leica SP5 upright *(room i04.U1.019): RETIRED
2. Leica SP5 inverted*(room i04.U1.019): book in PPMS
3. Leica SP8 inverted + FALCON (room i04.U1.015): book in PPMS
4. Leica SP8 upright + FALCON (room i04.U1.015): book in PPMS
5. Zeiss LSM800-1 inverted (room i06.U1.405): book in PPMS
6. Zeiss LSM800-2 upright + Airyscan (room i06.U1.403): book in PPMS
7. Zeiss LSM800-3 inverted (room i06.U1.406): book in PPMS
8. Zeiss LSM800-4 inverted + Airyscan (room i04.U1.018): book in PPMS
9. Zeiss LSM800-5 vertical 1 (room i04.O3.014): book in PPMS
10. Zeiss LSM800-6 Vertical 2 + Airyscan (room i04.O3.014): book in PPMS
11. Zeiss LSM880 inverted + Fast Airyscan (room i06.U1.406): book in PPMS
12. Zeiss LSM880 Upright + Airyscan (room i06.U1.403): book in PPMS
13. Zeiss LSM900-1 upright + Airyscan 2 (i04.U1.015): book in PPMS

03. Multiphoton Microscopes

1. LaVision TRIMscope MP inverted + FLIM (room i04.U1.019): book in PPMS
2. LaVision TRIMscope MP upright (room i04.U1.018): RETIRED
3. Leica Sp8 DIVE Multiphoton + confocal (room i04.U1.018) : book in PPMS

04. Spinning Disk

1. Andor SD & laser cutter microscope (room i04.U1.019): book in PPMS
2. Nikon CSU-W1-02 (room i04.U1.015): book in PPMS – Coming soon (2021)

05. Multi-modal multi-point confocal

• Andor Dragonfly 505 (room i04.U1.015): book in PPMS
• Nikon CSU-W1-01 Sora (room i04.U1.015): book in PPMS

06. TIRF Microscopes

1. Olympus IX83 TIRF (room i06.O2.031): book in PPMS
2. Visitron iLAS2 TIRF + FRAP (room i04.U1.018): book in PPMS
3. TILL Photonics SD & TIRF (room i06.U1.403): book in PPMS

07. Light sheet Microscopes

• OpenSPIM Light Sheet Microscope (room i06.U1.405): RETIRED

08. Force probing equipment

• JPK CellHesion Atomic Force Microscope (room i06.U1.405): book in PPMS

09. Stereo Microscopes (No Charge);

1. Leica stereo microscope (room i04.U1.019): book in PPMS
2. Olympus Stereo BFB (room i04.U1.018): book in PPMS
3. Olympus Stereo LBE (room i06.U1.403): book in PPMS
4. Zeiss Stereo Discovery (room i06.U1.406)

IMAGE ANALYSIS

10. Workstations: specifications (Image Analysis);

•  Analysis Workstation 01 (I01.O3.052b): book in PPMS
•  Analysis Workstation 02 (I01.O3.052b): book in PPMS
•  Analysis Workstation 03 (I01.O3.052b): book in PPMS
•  Analysis Workstation 04* (I01.O3.052b): book in PPMS
•  Analysis Workstation 05 (I01.O3.052b): book in PPMS
•  Analysis Workstation 06 (I01.O3.052b): book in PPMS
•  Analysis Workstation 07 (I01.O3.052a): book in PPMS
•  Analysis Workstation 08 (I01.O3.052a): book in PPMS
•  Analysis Workstation 09 (I01.O3.052a): book in PPMS
•  Analysis Workstation 10 (I01.O3.052a): book in PPMS
•  Analysis Workstation 11 (I01.O3.052a): book in PPMS
•  Analysis Workstation R1 (remote): book in PPMS – machine learning

11. Software: more info (€ = licensed);

• arivis Vision4D (1x float) – €
• Bitplane Imaris (8x float) + clearview (1x) + stitcher (1x) – €
• Fiji / Image J
• FlowJo – €
• Ilastik
• Imspector – €
• Mathworks matlab – €
• SVI Huygens (2x float) – €
• Wolfram Mathematica
• Zeiss ZEN (v2.1 / v2.3 /  v2.5 / v3.1) – €

AUTOMATED RENTAL SYSTEM

12. Accessories:

• Camera, peristaltic pump, Thermometer → info (location: i04.U1.Rentals)

13. Optical Tools:

• power meter, spectral meter, wavefront sensor→ info (location: i04.U1.Rentals)

14. Rental High-end objectives:

• Leica, Nikon, Olympus, Zeiss → info (location: i04.U1.Rentals)

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ii. EQUIPMENT PER BUILDING

• IST Austria Campus_Map

i01 – central building

• i01.O3.052a/b: image analysis workstations 2,3,4,6,7

i04 – Berthalanffy Foundation Building (BFB)

• i04.EG.018: BD Aria III | BD Canto II | Sony SH800
• i04.EG.035: Bioimaging Facility office BFB
• i04.UG.015: Andor Dragonfly 505 | Leica SP8inv-FALCON-WLL| Leica SP8up-FALCON-WLL |Zeiss LSM900-01 up | …
• i04.UG.018: Neurolucida | Zeiss LSM800-04 | Nicon Eclipse | Visitron iLAS TIRF | LaVision-2P up
• i04.UG.019: Andor SD – Lasercutter | LaVision-2P inv | Leica SP5 up* | Leica SP5 inv*
• i04.O2.031: Olympus TIRF
• i04.O3.014: Zeiss LSM800-05 vert1 | Zeiss LSM800-06 vert2

i06 – Lab Building East (LBE)

• i06.U1.107: Bioimaging Facility office LBE
• i06.U1.308: image analysis workstations 5,8,9
• i06.U1.403: Zeiss LSM800-02 up | Zeiss LSM880 up | Till Photonics / iMIC
• i06.U1.405: Zeiss LSM800-01 inv | openSPIM | JPK AFM
• i06.U1.406: Zeiss LSM800-03 inv | Zeiss LSM880 inv | Nikon Ti2 | Olympus slidescanner

CO3 –  Multi-purpose Hall

• CO3.EG.008a: Equipment demonstration and teaching lab

i23 –  Lab5 / Sandsone Building

• i23.u1.015: under construction (planned completion 2021, Q4)

i24 –  Lab6

• 23.u1: planing phase (planned completion 2023)

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* System has been replaced, and will be available for only limited remaining time

* RETIRED: systems not available – page kept for system information