Zeiss LSM 880 Upright + Airy Scan

Confocal scanner head for 3 descanned spectral channels, climate chamber

Location: room i06.U1.403

Specification: Zeiss Axio Examiner Z1 upright microscope

Detectors:

  1. 1 x GaAsP PMT
  2. 2 x multialkali PMTs
  3. 1 x transmitted light (T-PMT) detector
  4. 1 x AiryScan super-resolution detector

Illumination / excitation:

  1. Argon laser lines: 458nm, 488nm, 514nm
  2. Violet (405nm)
  3. green (561nm) and red (633nm) Diode lasers
  4. CoolLED lamp (visual inspection)
  5. Halogen (visual inspection)

Objectives:

1: Plan-Apochromat 10x / NA 0.45 Air (WD=2.1mm)
2: Plan-Apochromat 20x / NA 0.8 Air
3: Plan-Apochromat 20x / NA 1.0 Water DIC[421452-9900-000 ]4: Plan-Apochromat 40x / NA 1.2 Water
5: Plan-Apochromat 63x / NA 1.4 Oil

Modalities: ZEN black v2.3

  1. Fluorescence Correlation Spectroscopy (FCS) option.
  2. ZEN Module:  Experiment Designer: time series, multitime series, Z-stacks, tiles/multiposition images and channels in any order
  3. ZEN Module: Tiles & Positions: automatic scanning of predefined sample areas, plus acquisition of images according to a position list. Supports focus correction maps*, stitching and shading correction.
    Compatible with software autofocus.

Computer: HP Z820 Workstation,

  • 4 x 4GB RAM,
  • 128 GB SSD,
  • 2 x 2 TB SATA 7200 upm (RAID)

Contact persons:

Manual

See detailed description and manual: Zeiss LSM880 upright with Airy Scanner(pdf)

See Airyscan manual: How to set up Airyscan / Fast Airyscan(pdf)

Laser safety: https://ppms.ist.ac.at/vdoc/?pf=2&docid=50