Zeiss LSM 880 Inverted + Fast Airyscan

Confocal scanner head for 3 descanned spectral channels

Location: room I06.U1.406

specification: Zeiss Axio Observer Z1inverted microscope from Zeiss (Germany)

Software: ZEN black edition 2.3 SP1 + ZEN blue edition 2.3 (for image processing only!!!)

  1. one GaAsP PMT
  2. two multialkali PMTs
  3. A transmitted light (T-PMT) detector.
  4. An AiryScan super-resolution detector with Fast Airyscan option.

Illumination / excitation:

  1. Argon laser lines: 458nm, 488nm, 514nm
  2. Violet (405nm)
  3. green (561nm) and red (633nm) Diode lasers
  4. Cool LED (visual eximination)
  5. Halogen (visual eximination)

Sample conditioning:

  1. Temperature chamber
  2. Gas Incubation system for CO2 + O2 available from Ibidi (Germany)

Objectives:

Pos.1 Plan-Apochromat 10x / NA 0.45 Air (WD=2.1mm)

Pos.2 Plan-Apochromat 20x / NA 0.8 Air

Pos.3 Plan-Apochromat 40x / NA 1.3 OIL

Pos.4 Plan-Apochromat 63x / NA 1.4 Oil

Pos.5 LD C-Apochromat 40x / NA 1.1 Water

Pos.6 C-Apochromat 63x / NA 1.2 Water

Modalities

  1. Multiple position imaging

PC:

  • Intel® Xeon® CPU E5-2643 0 @ 3.3GHz
  • 80GB RAM
  • 1.81TB data drive

Contact persons:

Manuals:

See detailed description and manual: LSM880 inverted manual

See detailed description and manual for Airyscan/Fast Airyscan: How to set up Airyscan/Fast Airyscan

Booking: system in PMS SSU portal  

Laser safety: Document in PMS SSU portal