Zeiss LSM 800-02up_Airyscan

Location: I06.U1.403

Axio Imager.Z2 Upright LSM800 confocal microscope + Airyscan, suited for live imaging: equipped with heating chamber, CO2 + O2 mixer, ZEN 2.6 with experiment designer and macro-enviroment (OAD)

This confocal microscope is well suited for life imaging: book in PPMS– 4h limit in core hours

Overnight acquisitions: Please reboot system before and start system in DEBUG MODE (+ screenshot script): do not forget to SAVE the debug log-file in case of a crash!!

Sample conditioning:

  • Heating chamber
  • NO CO2 mixer

Light sources:

  • Laser lines 405, 488, 561, 640 nm
  • CoolLED p300 SB light
  • ESID LED

Detectors: 3 channels simultaneously (more sequentially)

  • 2x GaAsP PMTs: Gallium arsenide phosphide photomultiplier tube detectors with free choice of the spectral range. (Uses a tuneable short-pass and band-pass filter in front of the PMTs)
  • 1 x Airyscan detector – optimized for 63x. Can also be used as 3rd GaAsP detector!
  • 1x ESID+LED light source: Electronically switchable illumination and detection module

Motorized stage for multiple position imaging

  • Motorized stage for multiple position imaging
  • Multi-slide holder, dish holder, single slide holder

Objectives (M27 thread)

  1. Plan APOCHROMAT 20x/0.8 (420650-9901), WD=0.55 mm (D=0.17 mm)
  2. Plan-Apochromat 10x/0.45 (420640-9900), WD=2.1 mm
  3. Plan-Apochromat 40x/1.3 Oil DIC, UV-IR (420762-9800-799), WD=0.20 mm (Airyscan)
  4. Plan-Apochromat 20x / NA 1.0 W DIC (WD=1.8mm), (UV) VIS-IR

Not mounted objectives:

  • Objective Plan-Apochromat 63x / NA 1.4 Oil
  • Plan APOCHROMAT 40x/1.1 Water
  • Plan APOCHROMAT 5x/0.16

Software: Zen 2.6/ HotFix8

  1. ZEN Module Experiment Designer Hardware
  2. ZEN Module Tiles & Positions
  3. ZEN Macro Editor

Computer: HP Z6

  • Intel® Xeon™ E5-2623 v3 CPU @ 3.0GHz + 2GHz (2 processors)
  • 64GB RAM
  • 3.38TB data drive

Contact persons:

Manual:

See detailed description and manual:LSM800 upright-02 MANUAL_2019_08_06