Zeiss LSM 800-01 inverted

Location: i06.U1.405 (LBE)

Axio Observer.Z1 Inverted LSM800 confocal microscope, suited for live imaging: equipped with heating chamber, CO2 + O2 mixer, ZEN 2.3 with experiment designer. This confocal microscope is well suited for life imaging book in PPMS – 4h limit in core hours

Overnight acquisitions: Please reboot system before and start system in DEBUG MODE (+ screenshot script): do not forget to SAVE the debug log-file in case of a crash!!

Sample conditioning:

Light sources:

  • Laser lines 405, 488, 561, 640 nm
  • CoolLED p300 SB light
  • ESID LED

Detectors: 2 channels simultaneously (more sequentially)

  • 2x GaAsP PMTs: Gallium arsenide phosphide photomultiplier tube detectors with free choice of the spectral range. (Uses a tuneable short-pass and band-pass filter in front of the PMTs)
  • 1x ESID+LED light source: Electronically switchable illumination and detection module

Motorized stage for multiple position imaging

  • Multi-slide holder
  • Dish holder
  • Single slide holder

Objectives (M27 thread):

  1. Plan APOCHROMAT 20x/0.8 (420650-9901), WD=0.55 mm (D=0.17 mm)
  2. Plan-Apochromat 10x/0.45 (420640-9900), WD=2.1 mm
  3. Plan-Apochromat 40x/1.2 Water, WD=0.28 mm
  4. Plan-APOCHROMAT 63x/1.4 Oil
  5. Plan-APOCHROMAT 40x/1.4 Oil, DIC, WD=0.13

Software: Zen 2.3

  1. ZEN Module Experiment Designer Hardware
  2. ZEN Module Tiles & Positions

Computer: PC Esprimo P920

  • Intel® Core™ i5-4670 CPU @ 3.4GHz
  • 32GB RAM
  • 1.81TB data drive

Contact persons:

Manual:

See detailed description and manual:LSM800 MANUAL