Nikon Eclipse Ti-2E inverted

Widefield fluorescent microscope with incubation chamber and CO2 mixer available

book in ppms

Location: i06.U1.406

Specifications:

  • Emission filters
    • DAPI (LP 409, BP 447/60),
    • GFP (LP 495, BP 520/35),
    • mCherry (LP 596, BP 641/75),
    • FarRed – Cy5 (LP 669, BP 692/40),
    • TripleBand Spectra X (DAPI,GFP, mCherry),
    • CFP (LP 458, BP 483/32) – available on request, contact BIF 24h prior to slot
  • Lumencor light source with flexible excitation band selection:
    • Violet (390/18),
    • Blue (438/24),
    • Cyan (475/28),
    • Teal (513/17),
    • Green (549/15),
    • Yellow (575/25),
    • Red (632/22)
  • Timelapse option, motorized stage
  • Perfect Focus System (PFS)
  • DS-Qi2 monochrome camera
  • DIC imaging with 10x, 20x and 40x objectives
  • Nikon JOBS software: automation of acquisition

Objectives:

  • Plan Fluor 4x/0.2 air,
  • Plan Fluor 10x/0.3 DIC 1 N1 air PFS
  • Plan Apo λ 20x/0.5 DIC 1 air PFS
  • Plan Apo λ 40x/0.95 DIC air PFS

Contact persons:

Manuals

Resources:

To open ND2-files in FIJI: please go to Plugins/Bio-Formats/Bio-Formats Plugins Configuration under Formats you have to find Nikon ND2 (it is alphabetically sorted) and disable Use chunkmap table.

In case this doesn’t work, please try to enable Nikon’s reader or different combinations of the last 2 check boxes