Nikon Ti2E-01 inverted

Widefield fluorescent microscope with incubation chamber and CO2 mixer available

book in ppms

Location: i06.U1.406

Specifications:

  • Emission filters
    • DAPI (LP 409, BP 447/60)
    • GFP (LP 495, BP 520/35)
    • mCherry (LP 596, BP 641/75)
    • FarRed – Cy5 (LP 669, BP 692/40)
    • TripleBand Spectra X (DAPI,GFP, mCherry)
    • CFP (LP 458, BP 483/32) – available on request, contact BIF 24h prior to imaging time slot
  • Lumencor light source with flexible excitation band selection:
    • Violet (390/18)
    • Blue (438/24)
    • Cyan (475/28)
    • Teal (513/17)
    • Green (549/15)
    • Yellow (575/25)
    • Red (632/22)
  • Timelapse option, motorized stage
  • Perfect Focus System (PFS)
  • DS-Qi2 monochrome camera
  • DIC imaging with 10x, 20x and 40x objectives
  • Nikon JOBS software: automation of acquisition

Objectives:

  • Plan Fluor 4x/0.2 air
  • Plan Fluor 10x/0.3 DIC 1 N1 air PFS
  • Plan Apo λ 20x/0.5 DIC 1 air PFS
  • Plan Apo λ 40x/0.95 DIC air PFS

Contact persons:

Manuals:

Resources:

To open ND2 files in FIJI: go to Plugins > Bio-Formats > Bio-Formats Plugins Configuration. Under the tab Formats find Nikon ND2 (it is sorted alphabetically) and disable Use chunkmap table.

If this does not work, try enabling the Nikon reader or different combinations of the last 2 check boxes.