Leica SP5 Inverted

System has been replaced  – please move ongoing expermiments to new alternatives

Location

room: i04.U1.019:

Imaging modalities/features:

Specification:

  • DMi reseache microscope
  • TCS scan unit
  • Motorised Scanning stage
  • Super Z Galvo Stage Type H
  • Heated stage and objective heater (Pecon)

Light sources:

  • p100 white, LED Light source (Coolled)
  • Argon Laser lines 458, 488, 514,
  • DMOD 561 nm
  • DMOD 633 nm

Filters

  • DAPI: Excitation: BP 350/50 Emission: BP 460/50
  • FITC: Excitation: BP 480/40 Emission: BP 527/30
  • RHOD: Excitation: BP 546/10 Emission: BP 585/40

Objectives (M25 thread):

  1. HC PL APO 20x/0.7 CS (11506513), WD=0.59 mm, D=0.17 mm
  2. HCX PL APO 40x/1.25-0.75 OIL CS (11506251), WD=0.1 mm, D=0.17 mm
  3. HCX PL APO 63x/1.4-0.6 OIL CS (11506188), WD=0.1 mm, D=0.17 mm
  4. HCX PL APO 63x/1.1 W CORR CS (11506279), WD=0.22 mm, D=0.14-0.18 mm)
  5. HC PLAN APO 10x/0.4 0.17/A2.2 (11506165), WD=2.2 mm, D=0.17 mm
  6. HC PL IRAPO 40x/1.10 W CORR, WD=0.63, D=0.14-0.18 mm

Detection Filters:

  • ­NF 445
  • NF 488
  • NF 514
  • NF 458/514
  • NF 488/561/633 ­
  • NF 594
  • NF 445/594

Detectors:

  • 3x PMTs (free selection of detection range)
  • 2x HyD SMD (free selection of detection range)
  • 1 x Transmitted light detector (TLD)

Contact persons:

Manual

Detailed description and manual: Leica_SP5_Inverted_confocal_microscope manual.pdf