iMIC – SD/TIRF

Location: I06.U1.403 (LBE)

Inverted Spinning Disc microscope:

360° TIRF (Total Internal reflection)-microscopy with all laser lines possible
Pinhole size optimized for 60x objective

Laser lines: 405, 445, 488, 561, 640 nm

Emission filters:
Quad emission filter 445/40, 523/40, 600/40, 670/40
YFP filter 535/50
CFP filter 510/84

Objectives:

  1. 20x/0.75 dry,
  2. 40x/1.15 W imm,
  3. 60x/1.35 oil imm (for SD imaging),
  4. Olympus 60x/1.49 (for TIRF imaging)

Modalities

  1. multi-position (Motorized stage)
  2. Spinning disk
  3. TIRF

Sample handling:

  1. Temperature chamber
  2. CO2 mixer available

Contact persons:

Manual: iMic Spinning disc TIRF FRAP