Leica SP8 up (WLL,FALCON)

Location:

Room i04.UG.015 – 

Imaging modalities/features:

  • Confocal + real-time deconvolution (Lightning)
  • time gated acquisition
  • life time imaging
  • spectral unmixing
  • excitation Λ / emission λ wavelength scans

Specification:

  • DM6 CS 5 Research microscope
  • TCS SP8 FALCON
  • Motorised Scanning stage

Light sources:

  • Lumencor SOLA­ SM II, LED Light source
  • 405 nm laser: DMOD (50 mW) – Continuous wave
  • White Lite Laser (470-670 nm) Supercontinuum pulsed laser (max. 80 MHz): tunable in steps of 1 nm –  allows simultaneous use of up to 8 laser lines from UV to IR (8 channel AOTF: rapid modulation of laser intensity, particularly for ROI) –

Filter for visual examination:

    • DAPI: Excitation: BP 360/40 Emission: BP 470/40
    • FITC: Excitation: BP 470/40 Emission: BP 525/50
    • RHOD: Excitation: BP 546/10x Emission: BP 585/40

Objectives:

    1. HC PL Fluotar 5x / 0.15 (# 15506224), WD 12.0 mm – no immersion
    2. HC L Fluotar 16x / 0.60 MI (# 155065), WD 2.5 mm – ne 1.33-1.52 (water, silicon- or glycerol  immersion – (Correction collar for use with 0.0­ – 0.17 mm coverglass) + BABB cleared samples
    3. HC PL APO  20x / 0.75 MI (# 15506343), WD 0.67 mm – multi-immersion (CS2): water, glycerine and immersion oil
    4. HC FUOTAR L 25x / 0.95 W (# 15506374), WD=2.5 mm, wide angle (41°)

Detection Filters:

  • ­NF 445
  • NF 488
  • ­NF 514
  • NF 458/514
  • NF 488/561/633 ­
  • NF 594
  • NF 445/594

Detectors: 4 channel SP + BF

  1. PMT
  2. HyD SP GaAsP­Detektor for gated imaging
  3. PMT
  4. HyD2  SMD: specially selected for Falcon FLIM)
  5. Transmitted light detector (TLD)

Computer (CUDA Workstation):

  • Intel 6134 Xeon 3.2 8C
  • 192 GByte RAM
  • NVIDIA Quadro GP100 16GB Reference GPU (3584 cores) ­
  • 256 GB SATA SSD
  • 6 TByte SATA hard disk drive
  • 2 TB SSD RAID 0
  • 10Gb network card

Modules:

  • LAS X Live Data Mode
  • LAS X Dye Finder
  • LAS X 3D Visualisation
  • LAS X Phasor FLIM online
  • LAS X LIGHTNING Expert: Confocal Super­Resolution 

Contact persons:

Manual

Detailed description and manual